Cullin-7 Antibody

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Immunoprecipitation: Cullin-7 Antibody [NB100-539] - Detection of Human Cul7on HeLa whole cell lysate using NB100-539. Cul-7 was also immunoprecipitated using (NB100-538). Immunoprecipitation was also performed using ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-539
    • Availability
      Product Discontinued

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Cullin-7 Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 1650 and the C-terminus (residue 1698) of human Cullin homolog 7 using the numbering given in SwissProt entry Q14999 (GeneID 9820).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CUL7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: Does not perform well in WB. Use antibody NB 100-538 for WB. Immunoprecipitation: 2 to 5 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cullin-7 Antibody

  • CUL-7
  • cullin 7
  • cullin-7
  • KIAA0076dJ20C7.5

Background

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyze polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved in proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CUL7
Entrez
Uniprot