Human CTLA-4 ELISA Kit (Colorimetric)

Images

 
ELISA: Human CTLA-4 ELISA Kit (Colorimetric) [NBP1-91268] - CTLA-4 expression in human lung cancer cell lines determined by ELISA. ELISA image submitted by a verified customer review.
ELISA: Human CTLA-4 ELISA Kit (Colorimetric) [NBP1-91268] - Representative standard curve for human sCTLA-4 ELISA. Human sCTLA-4 was diluted in serial 2-fold steps in Sample Diluent.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.13 ng/mL

Order Details

Human CTLA-4 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CTLA-4 positive serum. There was no crossreactivity detected.
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.13 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10.40%
Intra-Assay
CV% < 4.80%
Spike Recovery
78%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CTLA4

Applications/Dilutions

Dilutions
  • ELISA
Reviewed Applications
Read 1 Review rated 5
using
NBP1-91268 in the following application:

Publications
Read Publications using
NBP1-91268 in the following applications:

  • 2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCTLA-4
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human sCTLA-4 monoclonal antibody
  5. Human sCTLA-4 Standard lyophilized, 20 ng/mL upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human CTLA-4 ELISA Kit (Colorimetric)

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), also known as CD152, is a cell surface glycoprotein belonging to the immunoglobulin family with a role in regulation of T cell activation (1). Human CTLA-4 is a 223 amino acid (aa) protein with a theoretical molecular weight of 24.6 kDa containing a leader peptide, a IgV-like domain, a transmembrane domain, and a cytoplasmic tail (1,2). CTLA-4 is both structurally and functionally related with another member of the immunoglobulin-related receptor family, CD28 (1-3). CTLA-4 and CD28 receptors are both expressed by CD4+ and CD8+ T cells and share two common ligands, CD80 (B7.1) and CD86 (B7.2), expressed on the surface of antigen presenting cells (APCs) (2,3). While CD28 is present on the plasma membrane of T cells, CTLA-4 is predominantly expressed intracellularly on vesicles in FoxP3+ regulatory T (Treg) cells and activated T cells due to endocytosis (3). While they share ligands, the two receptors have opposing functions in T cell activation; CD28 is involved in activation of T cells, while CTLA-4 functions as a negative regulator of T cell response (2,3). One of the primary functions of CTLA-4 is preventing autoimmunity (1-4).

Similar to programmed cell death protein 1 (PD-1), CTLA-4 is an inhibitory immune checkpoint protein (3,5). Checkpoint blockade immunotherapy using drugs or antibodies to target CTLA-4 is one of the main approaches for cancer treatment (5). A number of drugs targeting CTLA-4, or a combination of CTLA-4/PD-1, have been approved for treatment of various cancers like melanoma, renal cell carcinoma, and colorectal cancer (5). While blocking CTLA-4 in the tumor microenvironment is a promising cancer therapeutic, the absence of CTLA-4 under normal conditions can have deleterious effects. Studies have found that patients with CTLA-4 deficiency or mutations have clinical features associated with autoimmunity and immune dysregulation (4). Treatment options for CTLA-4 deficiency includes immunoglobulin-replacement therapy, corticosteroids, CTLA-4-immunoglobulin (Ig) fusion protein, and, in life-threatening cases, hematopoietic stem cell transplantation (4,6). Additionally, engaging CD80/CD86 with CTLA-4-Ig is a common immunosuppressive treatment for rheumatoid arthritis and kidney transplant recipients (6).

References

1. Romo-Tena, J., Gomez-Martin, D., & Alcocer-Varela, J. (2013). CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmunity reviews, 12(12), 1171-1176. https://doi.org/10.1016/j.autrev.2013.07.002

2. Hosseini, A., Gharibi, T., Marofi, F., Babaloo, Z., & Baradaran, B. (2020). CTLA-4: From mechanism to autoimmune therapy. International immunopharmacology, 80, 106221. https://doi.org/10.1016/j.intimp.2020.106221

3. Rowshanravan, B., Halliday, N., & Sansom, D. M. (2018). CTLA-4: a moving target in immunotherapy. Blood, 131(1), 58-67. https://doi.org/10.1182/blood-2017-06-741033

4. Verma, N., Burns, S. O., Walker, L., & Sansom, D. M. (2017). Immune deficiency and autoimmunity in patients with CTLA-4 (CD152) mutations. Clinical and experimental immunology, 190(1), 1-7. https://doi.org/10.1111/cei.12997

5. Rotte A. (2019). Combination of CTLA-4 and PD-1 blockers for treatment of cancer. Journal of experimental & clinical cancer research : CR, 38(1), 255. https://doi.org/10.1186/s13046-019-1259-z

6. Bluestone, J. A., St Clair, E. W., & Turka, L. A. (2006). CTLA4Ig: bridging the basic immunology with clinical application. Immunity, 24(3), 233-238. https://doi.org/10.1016/j.immuni.2006.03.001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
202-IL
Species: Hu
Applications: BA
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
AF1107
Species: Mu
Applications: IHC, WB
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
MAB342
Species: Hu
Applications: AgAct, WB
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
D6050
Species: Hu
Applications: ELISA
6507-IL/CF
Species: Hu
Applications: BA
AF2009
Species: Hu
Applications: ICC, IHC
DY417
Species: Mu
Applications: ELISA
NB100-77417
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, In vivo, WB
NBP2-67605
Species: Hu, Pm, Mu
Applications: ICC/IF, IHC, IHC-P, IP, ISH, WB
DR2A00
Species: Hu
Applications: ELISA
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC

Publications for CTLA-4 ELISA Kit (NBP1-91268)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA, WB.


Filter By Application
ELISA
(2)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species

Review for CTLA-4 ELISA Kit (NBP1-91268) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-91268:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
ELISA CTLA-4 NBP1-91268
Enlarge
5
reviewed by:
Christopher England
ELISA Human 03/10/2016
View

Summary

ApplicationELISA
Sample TestedHuman lung cancer cell lines
SpeciesHuman
Lot122662027

Blocking

Blocking DetailsThe exact protocol provided by Novus for this antibody kit was followed. No blocking details.

Primary Anitbody

Dilution RatioWe did not have to dilute our samples for ELISA. We only normalized the total protein to 20 ug.

Details

Detection NotesThe plate was read at 450 nm using the Biotek Synergy 4 plate reader.
ELISA Plate DetailsThe kit contained a plate pre-coated with sCTLA-4 antibody
Wash Description400 uL of the provided wash buffer was used to wash wells. We would let it sit for 10 s before aspiration. Washed 4-5X.

Comments

CommentsMake sure to read the protocol at least once all the way through before starting the ELISA. It only takes about 4-5 hours total time. Also, our standard curve did not correlate well with the standard curve provided in the protocol book (Yes, a 40 page book that is very detailed), so don't be surprised if you standard curve is off too. Our standard curve provided an r squared value > 0.98, so everything turned out great. Really liked the kit and will use it again in the future.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CTLA-4 ELISA Kit (NBP1-91268). (Showing 1 - 1 of 1 FAQs).

  1. Does this kit work with CTLA4Ig fusion protein? CTLA4Ig contains the extracellular domain of human CTLA4 and human IgG1 Fc. Do you know if any customers use this kit to check CTLA4Ig? sCTLA4 and CTLA4Ig have the same extracellular domain. If the two antibodies used in the ELISA kit are against the extracellular domain, it could be used to detect CTLA4Ig. Could you please tell me the epitopes of the two antibodies?
    • The standard protein in our human CTLA-4 is a soluble fusion protein consisting of the extracellular domain of human CTLA-4, fused to mouse IgG2a Fc. This means that our kit will detect your CTLA4Ig.

Additional CTLA-4 Products

Bioinformatics Tool for CTLA-4 ELISA Kit (NBP1-91268)

Discover related pathways, diseases and genes to CTLA-4 ELISA Kit (NBP1-91268). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CTLA-4 ELISA Kit (NBP1-91268)

Discover more about diseases related to CTLA-4 ELISA Kit (NBP1-91268).
 

Pathways for CTLA-4 ELISA Kit (NBP1-91268)

View related products by pathway.

PTMs for CTLA-4 ELISA Kit (NBP1-91268)

Learn more about PTMs related to CTLA-4 ELISA Kit (NBP1-91268).
 

Research Areas for CTLA-4 ELISA Kit (NBP1-91268)

Find related products by research area.

Blogs on CTLA-4.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated ...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Christopher England
03/10/2016
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol CTLA4