Recombinant CTGF/CCN2 Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-43178
    • Availability
      Product Discontinued

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Recombinant CTGF/CCN2 Protein Summary

Description
A partial protein corresponding to amino acids Lys 254 - Ala 349 of CTGF (CAG46534).

Source: E. coli

Preparation
Method
E. coli-expressed amino acids Lys251- Ala349 (Accession # CAG46534).
Details of Functionality
This reagent has been tested in bioassays using the HUVEC cell line. The ED50, as determined by the dose-dependent induction of HUVEC cell proliferation, is 1.6 ug/ml, corresponding to a specific activity of 6 x 10^2Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
CTGF
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Theoretical MW
11.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid, Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant CTGF/CCN2 Protein

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for CTGF/CCN2 Partial Recombinant Protein (NBP1-43178). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

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Bioinformatics

Gene Symbol CTGF
Entrez