CTCF Antibody


Immunoprecipitation: CTCF Antibody [NB500-194] - Immunoprecipitation of Human CTCF. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CTCF ...read more

Product Details

Reactivity HuSpecies Glossary
Applications IP, WB (-)
1.0 mg/ml

Order Details

CTCF Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 727) of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664)
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Rabbit (100%), Xenopus (100%), Primate (100%), Bovine (100%), Zebrafish (100%), Turkey (100%), Monkey (100%), Guinea Pig (100%), Chicken (100%), Canine (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
83 kDa
Read Publication using NB500-194.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Brown alga_2880 and Spotted green pufferfish.

Alternate Names for CTCF Antibody

  • 11 zinc finger transcriptional repressor
  • 11-zinc finger protein
  • 11-Zinc Finger Protein2
  • CCCTC-binding factor (zinc finger protein)
  • CCCTC-binding Factor
  • CTCFL paralog
  • transcriptional repressor CTCF


The vertebrate insulator protein, CCCTC binding factor (CTCF) is a highly conserved, ubiquitously expressed DNA-binding protein that has widespread capabilities in gene regulation. CTCF contains 11 zinc finger motifs and it is thought that CTCF uses different combinations of zinc fingers to recognise a variety of specific sequences on the DNA to control several important genes. CTCF plays dual roles in cell growth regulatory processes and epigenetic functions. CTCF is a negative regulator of cell proliferation and is located in a region of the chromosome that is frequently deleted in human cancers. The CTCF protein is altered in some breast and prostate tumours. Substitutions from one amino acid to another in the DNA-binding domain change the whole spectrum of the genes regulated by CTCF which can result in cancer development. CTCF was first identified as one of the nuclear factors binding to the regulatory regions of the c-myc gene. Further study of this protein revealed roles in transcriptional repression, insulator function, and imprinting genetic information. Among the multitude of normal CTCF functions, it's involvement in partitioning the genome into active or inactive domains by means of a chromatin insulator function has received particular attention. As an insulator CTCF helps to define the boundaries between the neighbouring genes. Strikingly, all vertebrate chromatin insulators identified so far interact with CTCF.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol CTCF

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