Crk Antibody

Images

 
Western Blot: Crk Antibody [NBP2-16013] - Western Blot analysis of CRK expression in transfected 293T cell line by CRK polyclonal antibody. A: Non-transfected lysate. B: CRK transfected lysate. 12% SDS PAGE gel, diluted ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.94 mg/ml

Order Details

Crk Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.
Localization
Cytoplasm; Cell membrane
Predicted Species
Mouse (99%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.94 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Crk Antibody

  • adapter molecule crk
  • avian sarcoma virus CT10 (v-crk) oncogene homolog
  • Crk
  • CRKII
  • p38
  • Proto-oncogene c-Crk
  • v-crk avian sarcoma virus CT10 oncogene homolog
  • v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRK
Entrez
Uniprot