Creatine Kinase B Recombinant Protein

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SDS-Page: Creatine Kinase B Recombinant Protein [NBP1-50842]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Creatine Kinase B Recombinant Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 381 of Human Creatine Kinase B

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Preparation
Method
E.coli
Gene
CKB
Purity
>95% pure by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 44.8 kDa (401aa), confirmed by MALDI-TOF

Alternate Names for Creatine Kinase B Recombinant Protein

  • B-CK
  • Creatine kinase B chain
  • creatine kinase, brain
  • creatine kinase-B
  • EC 2.7.3
  • EC 2.7.3.2

Background

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells. Recombinant human CKB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography technique

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CKB

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