Recombinant Human Creatine Kinase B Protein

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SDS-Page: Creatine Kinase B Recombinant Protein [NBP1-50842]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Creatine Kinase B Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 381 of Human Creatine Kinase B

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
CKB
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 44.8 kDa (401aa), confirmed by MALDI-TOF
Theoretical MW
44.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Creatine Kinase B Protein

  • B-CK
  • Creatine kinase B chain
  • creatine kinase, brain
  • creatine kinase-B
  • EC 2.7.3
  • EC 2.7.3.2

Background

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells. Recombinant human CKB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography technique

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CKB

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