Creatine Kinase B Antibody

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Western Blot: Creatine Kinase B Antibody [NB100-2847] - Analysis using NB100-2847 at 0.1 ug/ml in Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Creatine Kinase B Antibody Summary

Immunogen
Peptide with sequence C-KPSDEHKTDLNPDN corresponding to internal region according to NP_001814.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CKB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 45-48 kDa band observed in human brain lysates (calculated MW of 42.6 kDa band according to NP_001814.2).
Positive Control
Creatine Kinase B Lysate (NBL1-09218)
Publications
Read Publication using NB100-2847.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Creatine Kinase B Antibody

  • B-CK
  • Creatine kinase B chain
  • creatine kinase, brain
  • creatine kinase-B
  • EC 2.7.3
  • EC 2.7.3.2

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Creatine Kinase B Antibody (NB100-2847)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CKB
Entrez
Uniprot