Cre Antibody

Images

 
Western Blot: Cre Antibody [NBP2-16002] - A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins 12.5% SDS-PAGE Cre recombinase antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: Cre Antibody [NBP2-16002] - Cre antibody detects Cre protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of Cre-ERT2-transfected 293T cells ...read more
Immunoprecipitation: Cre Antibody [NBP2-16002] - IP Sample: 293T whole cell lysate/extract A : 20 ug whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 ug of pre-immune ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cre Antibody Summary

Immunogen
The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cre Antibody (NBP2-16002) (0)

There are no publications for Cre Antibody (NBP2-16002).
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cre Antibody (NBP2-16002). (Showing 1 - 1 of 1 FAQ).

  1. A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
    • With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

Secondary Antibodies

 

Isotype Controls

Additional Cre Products

Cre NBP2-16002

Blogs on Cre.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

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Calculators

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