COX4 Antibody

Images

 
Western Blot: COX4 Antibody [NB100-68150] - Staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.01ug/ml)
Immunohistochemistry-Paraffin: COX4 Antibody [NB100-68150] - Staining of Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. (2.5ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

COX4 Antibody Summary

Immunogen
Peptide with sequence C-QGLASKWDYEKNE corresponding to C-Terminus according to NP_001852.1.
Epitope
QGLASKWDYEKNE
Marker
Mitochondria Marker
Specificity
This antibody is expected to recognize COX4I1 (GeneID:1327; MW: 19.6kDa) & COX4I2 (GeneID:84701, MW:19.6kDa).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COX4I1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 18 kDa band observed in human skeletal muscle lysates (calculated MW of 19.6 kDa band according to NP_001852.1). IHC-P: Human thyroid gland shows pixulate (mitochondrial) staining in the cytoplasm of epithelial cells.
Positive Control
COX4 Lysate (NBL1-09409)
Publications
Read Publication using NB100-68150.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COX4 Antibody

  • COX IV-1
  • COX4
  • COX4-1
  • COX4COXIV
  • COX4I2
  • Cytochrome c oxidase polypeptide IV
  • cytochrome c oxidase subunit 4 isoform 1, mitochondrial
  • cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
  • MGC72016

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-68150
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Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for COX4 Antibody (NB100-68150)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for COX4 Antibody (NB100-68150)

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Blogs on COX4.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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Bioinformatics

Gene Symbol COX4I1
Entrez
Uniprot