Cortisol Antibody (XM210)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
XM210
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cortisol Antibody (XM210) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified cortisol with BSA.
Localization
Secreted.
Specificity
Cortisol, Ka = 1.7 x 10^9_ M-1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • ELISA

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Cortisol Antibody (XM210)

  • Compound F
  • Cortisol
  • Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Cortisol Antibody (NB200-427). (Showing 1 - 1 of 1 FAQ).

  1. Has your Cortisol monoclonal antibody (NB200-427) been tested for cross-reactivity against the following list of compounds? Corticosterone, Prednisone, Progesterone, Cortisone, 11-deoxycortisol, Prednisolone, Testosterone, Other.
    • We have tested NB200-427 against the following: 11-Deoxycortisol (0.9%), Prednisolone (5.6%), Corticosterone (0.6%), 11-Deoxycorticosterone (<0.1%), Progesterone (<0.1%), 17-Hydroxyprogesterone (<0.1%), Testosterone, Estradiol, Estriol (<0.1%), Danazol (<0.01%).

Secondary Antibodies

 

Isotype Controls

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