Connexin 43/GJA1 Antibody


Western Blot: Connexin 43/GJA1 Antibody [NBP1-19879] - extracts from mouse heart cells.
Immunohistochemistry-Paraffin: Connexin 43/GJA1 Antibody [NBP1-19879] - Paraffin-embedded human brain tissue.

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Connexin 43/GJA1 Antibody Summary

Synthetic peptide, corresponding to amino acids 330-380 of Human Connexin 43.
Gap Junctions Marker
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of Connexin 43 (R361) pAb in extracts from mouse heart cells.
Immunohistochemistry (IHC) analyzes of Connexin 43 (R361) pAb in paraffin-embedded human brain tissue.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Connexin 43/GJA1 Antibody

  • Connexin 43
  • connexin-43
  • CX43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3


The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the ab-sence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics Tool for Connexin 43/GJA1 Antibody (NBP1-19879)

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Blogs on Connexin 43/GJA1.

Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.

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Gene Symbol GJA1