Complement C3 Antibody



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Complement C3 Antibody Summary

Complement C3 native protein, sequence 1-1663 (NP_000055.1).
Immunogen affinity purified
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  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.49 mg/ml
Immunogen affinity purified

Alternate Names for Complement C3 Antibody

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product


The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Complement C3 Antibody (NB300-352). (Showing 1 - 2 of 2 FAQ).

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.
  2. Can I please ask you a question: NB300-352 is polyclonal and immunogen is the whole protein C3, but can you ensure me that antibody does recognize C3 and all its fragments (C3b, iC3b, C3c, C3dg and maybe the smallest: C3a and C3f)? The best would be to have a picture of a western blot to see which bands at which molecular weight do appear.
    • This product was developed and tested by one of our collaborating lab, and unfortunately, we do not have a western blot image to provide at the moment. Technically, the antibody should be able to detect the fragments as long as they can be resolved on the gel.

Secondary Antibodies


Isotype Controls

Additional Complement C3 Products

Bioinformatics Tool for Complement C3 Antibody (NB300-352)

Discover related pathways, diseases and genes to Complement C3 Antibody (NB300-352). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Complement C3 Antibody (NB300-352)

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Blogs on Complement C3.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Gene Symbol C3