Complement C3 Antibody (11H9)

Images

 
Biological Strategies: Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a ...read more
Immunocytochemistry/ Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in ...read more
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but ...read more
Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow ...read more
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
11H9
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Complement C3 Antibody (11H9) Summary

Immunogen
C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
Localization
Secreted
Specificity
Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
C3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/mL
  • Immunoassay 0.5 ug/well in PBS
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB200-540 in the following applications:

Publications
Read Publications using
NB200-540 in the following applications:

  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Complement C3 Antibody (11H9)

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product

Background

Complement component 3, or C3, is one of nine major complement proteins in the complement system. This system is vital to the immune system because of its role in killing unwanted bacteria and its part in the initiation of inflammation. The complement system is made up of a collection of proteins found in the bloodstream. C3 happens to be the most important protein as well as the most plentiful among the other proteins in the complement system. Levels of C3 in the blood are often tested in patients with autoimmune disorders to monitor the effects of their treatment. A C3 deficiency can cause increased risk for bacterial infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement C3 Antibody (NB200-540)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(2)
All Applications
Filter By Species
Mouse
(2)
All Species

Reviews for Complement C3 Antibody (NB200-540) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-540:
Filter by Applications
Flow
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry Complement C3 NB200-540
Enlarge
reviewed by:
Francesca Azar
Flow Mouse 05/16/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedBreast tumor
SpeciesMouse
LotAB042114A-06

Comments

CommentsDespite some issues with our secondary antibody, this antibody worked well for flow cytometry analysis of mouse tumor tissue.
 
reviewed by:
Anonymous
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for Complement C3 Antibody (NB200-540). (Showing 1 - 1 of 1 FAQs).

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-540AF350
Alexa Fluor 405 NB200-540AF405
Alexa Fluor 488 NB200-540AF488
Alexa Fluor 532 NB200-540AF532
Alexa Fluor 594 NB200-540AF594
Alexa Fluor 647 NB200-540AF647
Alexa Fluor 700 NB200-540AF700
Alexa Fluor 750 NB200-540AF750
Allophycocyanin NB200-540APC
Allophycocyanin/Cy7 NB200-540APCCY7
Biotin NB200-540B
DyLight 350 NB200-540UV
DyLight 405 NB200-540V
DyLight 488 NB200-540G
DyLight 550 NB200-540R
DyLight 594 NB200-540DL594
DyLight 650 NB200-540C
DyLight 680 NB200-540FR
DyLight 755 NB200-540IR
FITC NB200-540F
HRP NB200-540H
Janelia Fluor 549 NB200-540JF549
Janelia Fluor 646 NB200-540JF646
PE NB200-540PE
PE/Atto594 NB200-540PEATT594
PE/Cy5.5 NB200-540PECY55
PE/Cy7 NB200-540PECY7
PerCP NB200-540PCP

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Blogs on Complement C3.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Francesca Azar
05/16/2019
Application: Flow
Species: Mouse

 
Anonymous
04/01/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol C3
Entrez
Uniprot