COMP/Thrombospondin-5 Overexpression Lysate (Native)

Images

 
Western Blot: COMP Overexpression Lysate (Adult Normal) [NBP2-09056] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COMP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

COMP/Thrombospondin-5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000086. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens COMP, mRNA.
Gene
COMP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
80.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for COMP/Thrombospondin-5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for COMP/Thrombospondin-5 Overexpression Lysate (Native)

  • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
  • cartilage oligomeric matrix protein
  • cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
  • COMP
  • EDM1
  • EPD1
  • MED
  • MEDMGC131819
  • MGC149768
  • PSACH
  • pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
  • THBS5
  • Thrombospondin5
  • Thrombospondin-5
  • TSP5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for COMP/Thrombospondin-5 Lysate (NBP2-09056) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for COMP/Thrombospondin-5 Lysate (NBP2-09056). (Showing 1 - 1 of 1 FAQ).

  1. I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
    • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

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Bioinformatics

Gene Symbol COMP
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