Human COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

ELISA: COMP/Thrombospondin-5 ELISA Kit [HRP] [KA0021] - Standard curve

Product Discontinued

Human COMP/Thrombospondin-5 ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. COMP (Human) ELISA Kit is a double monoclonal sandwich enzyme immunoassay for the quantitative measurement of human COMP in serum or plasma.
• Standard Curve Range: 4 to 128 ng/mL
• Sensitivity: 0.4 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.80%
• Intra-Assay: CV% < 5.90%
• Spike Recovery: 91.30%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: COMP

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

• cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• cartilage oligomeric matrix protein
• cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• COMP
• EDM1
• EPD1
• MED
• MEDMGC131819
• MGC149768
• PSACH
• pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
• THBS5
• Thrombospondin5
• Thrombospondin-5
• TSP5

Background

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for COMP/Thrombospondin-5 ELISA Kit (KA0021)(5)

Publications using KA0021

• Roberts HM, Moore JP, Thom JM. The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females. Eur J Appl Physiol 2018-06-01 [PMID: 29536174] (ELISA, Human)
• Klocke R, Levasseur K, Kitas GD et al. Cartilage turnover and intra-articular corticosteroid injections in knee osteoarthritis. Rheumatol Int 2018-02-02 [PMID: 29396701]
• Georgiev T, Ivanova M, Kopchev A et al. Cartilage oligomeric protein, matrix metalloproteinase-3, and Coll2-1 as serum biomarkers in knee osteoarthritis: a cross-sectional study. Rheumatol Int 2017-11-21 [PMID: 29164307]
• Roberts HM, Moore JP, Griffith-McGeever CL et al. The effect of vigorous running and cycling on serum COMP, lubricin, and femoral cartilage thickness: a pilot study. Eur J Appl Physiol 2016-08-01 [PMID: 27251407]
• Law RJ, Saynor ZL, Gabbitas J et al. The Effects of Aerobic and Resistance Exercise on Markers of Large Joint Health in Stable Rheumatoid Arthritis Patients: A Pilot Study. Musculoskeletal Care 2015-12-01 [PMID: 25962747]

FAQs for COMP/Thrombospondin-5 ELISA Kit (KA0021). (Showing 1 - 2 of 2 FAQs).

1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
   • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374
2. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: COMP