COMP/Thrombospondin-5 Antibody (MA37C94 (HC484D1))

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Western Blot: COMP/Thrombospondin-5 Antibody (MA37C94 (HC484D1)) [NB100-64779] - Purifed COMP from conditioned media. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, IP
Clone
MA37C94 (HC484D1)
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

COMP/Thrombospondin-5 Antibody (MA37C94 (HC484D1)) Summary

Immunogen
Human cartilage derived COMP
Specificity
Recognizes human cartilage oligomeric matrix protein (COMP). The recognizes an epitope located in the central portion of the molecule.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
COMP
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Reviewed Applications
Read 1 Review rated 5
using
NB100-64779 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for COMP/Thrombospondin-5 Antibody (MA37C94 (HC484D1))

  • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
  • cartilage oligomeric matrix protein
  • cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
  • COMP
  • EDM1
  • EPD1
  • MED
  • MEDMGC131819
  • MGC149768
  • PSACH
  • pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
  • THBS5
  • Thrombospondin5
  • Thrombospondin-5
  • TSP5

Background

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COMP/Thrombospondin-5 Antibody (NB100-64779) (0)

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Review for COMP/Thrombospondin-5 Antibody (NB100-64779) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-64779:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot COMP/Thrombospondin-5 NB100-64779
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reviewed by:
Anonymous
WB Human 09/10/2014
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Summary

ApplicationWestern Blot
Sample TestedPurifed COMP from conditioned media
SpeciesHuman

Blocking

Blocking Details5% non fat milk 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 in blocking buffer, 1 hour at room temperature500

Secondary Antibody

Secondary DescriptionDonkey anti-Rat IgG-HRP

Details

Detection NotesECL, 30 seconds exposure

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for COMP/Thrombospondin-5 Antibody (NB100-64779). (Showing 1 - 1 of 1 FAQ).

  1. I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
    • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

Secondary Antibodies

 

Isotype Controls

Additional COMP/Thrombospondin-5 Products

Bioinformatics Tool for COMP/Thrombospondin-5 Antibody (NB100-64779)

Discover related pathways, diseases and genes to COMP/Thrombospondin-5 Antibody (NB100-64779). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for COMP/Thrombospondin-5 Antibody (NB100-64779)

Discover more about diseases related to COMP/Thrombospondin-5 Antibody (NB100-64779).
 

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Blogs on COMP/Thrombospondin-5

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Recent Reviews

5
5
1
4
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2
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1
0

Anonymous
09/10/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol COMP
Entrez
Uniprot