| Reactivity | ChisSpecies Glossary |
| Applications | Bioactivity, Enzyme Activity |
| Concentration | LYOPH |
| Description | Collagenases are enzymes that break the peptide bonds in collagen, and is useful in structural and functional studies of collagen metabolism. Collagenase Type 3 contains low levels of proteolytic activity. Source: Native protein purified from Clostridium histolyticum |
| Preparation Method |
Native protein purified from Clostridium histolyticum |
| Source | Clostridium histolyticum |
| Protein/Peptide Type | Native Protein |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
| Dilutions |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. Do not freeze. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
| Reconstitution Instructions | Primary cell isolation, culture and tissue dissociation: Reconstitute in balanced salt solutions at concentrations from 0.05% to 0.5% (w/v). Enzymetic assay: Reconstitute in 0.05 M TES buffer with 0.36 mM CaCl2, pH 7.5 (concentration of 1.0 mg/ml) |
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