Collagen VI Overexpression Lysate (Native)

Images

 
Western Blot: Collagen VI Overexpression Lysate (Adult Normal) [NBP2-10777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Collagen VI.

Product Details

Summary
Applications WB

Order Details

Collagen VI Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001839. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Collagen VI, mRNA.
Gene
COL6A1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
106.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Collagen VI

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Collagen VI Overexpression Lysate (Native)

  • Collagen 6
  • collagen alpha-1(VI) chain
  • collagen VI, alpha-1 polypeptide
  • collagen, type VI, alpha 1
  • Collagen-6
  • human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
  • OPLL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-90951
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-34837
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NBP2-36776
Species: Hu
Applications: WB, ICC/IF, IHC
AF1936
Species: Hu
Applications: WB, IP
NBP2-27561
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-30078
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P
NBP1-88119
Species: Hu
Applications: IHC, IHC-P
H00005688-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB600-408
Species: Hu, Mu, Rt, Po, Bv, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB1419
Species: Hu, Rt
Applications: IHC, ICC, ICFlow
NBP2-34234
Species: Hu
Applications: ICC/IF, IHC-Fr, IHC-P
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P
NB600-594
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB120-7164
Species: Hu, Mu, Rt, Fi
Applications: ELISA, ICC/IF, MiAr
NBP1-76289
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, ICC
NBP2-10777
Applications: WB

Publications for Collagen VI Lysate (NBP2-10777) (0)

There are no publications for Collagen VI Lysate (NBP2-10777).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Collagen VI Lysate (NBP2-10777) (0)

There are no reviews for Collagen VI Lysate (NBP2-10777). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Collagen VI Lysate (NBP2-10777) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Collagen VI Products

Related Products by Gene

Bioinformatics Tool for Collagen VI Lysate (NBP2-10777)

Discover related pathways, diseases and genes to Collagen VI Lysate (NBP2-10777). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Collagen VI Lysate (NBP2-10777)

Discover more about diseases related to Collagen VI Lysate (NBP2-10777).
 

Pathways for Collagen VI Lysate (NBP2-10777)

View related products by pathway.

PTMs for Collagen VI Lysate (NBP2-10777)

Learn more about PTMs related to Collagen VI Lysate (NBP2-10777).
 

Research Areas for Collagen VI Lysate (NBP2-10777)

Find related products by research area.

Blogs on Collagen VI

There are no specific blogs for Collagen VI, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol COL6A1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-10777 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought