Collagen V Antibody

Images

 

Product Details

Summary
Product Discontinued
View other related Collagen V Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50132
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Collagen V Antibody Summary

Immunogen
The antibody reacts with Human Collagen V.
Clonality
Polyclonal
Host
Rabbit
Gene
COL5A1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Recommended for use in immunohistochemistry on frozen cells and sections. Suitable for dot-blotting and ELISA on native human collagen type V. Use on paraffin sections is not tested.Antibodies can be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1:10.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1 ml IgG antiserum (0.1 mg/ml)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ammonium sulfate precipitation
Reconstitution Instructions
Reconstitute with 1 ml sterilized water and with preservative if preferred.

Alternate Names for Collagen V Antibody

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

Purified native collagen type V extracted from human skin into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, II, III and IV to remove cross-reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody is obtained by binding to immobilized native human collagen type V (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type V. Inhibition by other types of collagens is observed only at 20-50 times higher concentration. Characteristic immunostaining pictures of frozen sections of human kidney, liver, skin and heart are produced to certify batch quality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00001290-M02
Species: Hu
Applications: WB, ELISA, ICC/IF
NB600-408
Species: Hu, Mu, Rt, Po, Bv, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB600-594
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB110-68136
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
NBP2-58529
Species: Hu
Applications: ICC/IF
AF143
Species: Hu
Applications: WB, Simple Western, IHC
H00007134-M01
Species: Hu
Applications: WB, ELISA, IHC-P
NBP2-58159
Species: Hu
Applications: ICC/IF
NB600-844
Species: Hu, Mu, Rt, Ch, Rb
Applications: WB, IHC, IHC-Fr, IHC-P, IF
NBP1-88062
Species: Hu
Applications: WB, IHC, IHC-P
NB110-8146
Species: Hu, Po, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IM
NBP1-90306
Species: Hu
Applications: WB, IHC, IHC-P
NB100-74350
Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF6999
Species: Hu
Applications: WB, Simple Western, IHC
NBL1-11228
Species: Hu
NBP1-59069
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-48899
Species: Hu
Applications: IHC
NBP2-43728
Species: Hu
Applications: WB, ICC/IF
NBP1-50132
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-Fr

Publications for Collagen V Antibody (NBP1-50132) (0)

There are no publications for Collagen V Antibody (NBP1-50132).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Collagen V Antibody (NBP1-50132) (0)

There are no reviews for Collagen V Antibody (NBP1-50132). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Collagen V Antibody (NBP1-50132) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Collagen V Products

Bioinformatics Tool for Collagen V Antibody (NBP1-50132)

Discover related pathways, diseases and genes to Collagen V Antibody (NBP1-50132). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Collagen V Antibody (NBP1-50132)

Discover more about diseases related to Collagen V Antibody (NBP1-50132).
 

Pathways for Collagen V Antibody (NBP1-50132)

View related products by pathway.

PTMs for Collagen V Antibody (NBP1-50132)

Learn more about PTMs related to Collagen V Antibody (NBP1-50132).
 

Research Areas for Collagen V Antibody (NBP1-50132)

Find related products by research area.

Blogs on Collagen V

There are no specific blogs for Collagen V, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Collagen V Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol COL5A1