Collagen V Antibody

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Product Details

Summary
Product Discontinued
View other related Collagen V Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50126
    • Availability
      Product Discontinued

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Collagen V Antibody Summary

Immunogen
The antibody reacts with the Collagen V antigen.
Specificity
The antiserum was subjected to immune-absorption with immobilized human serum proteins to remove non-specific reacting antibodies. Collagen cross reactions, caused by the presence of antibodies to common antigenic determinants on various collagens were removed by immune absorption on immobilized collagens types I, II, III and IV. The specificity of the antibody was ascertained by competition ELISA. Complete inhibition was found if the antibody was preincubated with collagen type V. Minimal cross reactions with other collagens were only found if 5-10 times higher concentrations were used of other collagens. No reactions were found with fibronectin, fibrinogen and laminin. The specificity of the product was furthermore tested by immunohistochemistry on frozen sections of large series of human tissues. Characteristic immunostaining pictures of frozen sections of human kidney, liver, skin and heart are produced to verify the batch quality
Clonality
Polyclonal
Host
Rabbit
Gene
COL5A1
Purity
Ammonium sulfate precipitation
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Recommended for use in immunohistochemistry on frozen sections. Suitable for dot-blotting and ELISA on native human collagen V. Use on paraffin sections is not tested.Antibodies can be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1:10.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1 ml antiserum (0.2 mg/ml)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ammonium sulfate precipitation
Reconstitution Instructions
Reconstitute with 1 ml sterilized water and with preservative if preferred.

Alternate Names for Collagen V Antibody

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL5A1