Collagen V Antibody (NLI/64)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-62158
    • Availability
      Product Discontinued

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Collagen V Antibody (NLI/64) Summary

Immunogen
Purified human collagen V
Localization
Extracellular matrix
Specificity
NB100-62158 is specific for collagen V. It does not bind to human collagen types I,II,III and IV. In cryostat sections, stains all basement membranes (BMs). Overall pattern varies with exact protocol used. Useful for discriminating between malignant and benign neoplasms, the former showing apparent discontinuity of BM.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
COL5A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline.
Preservative
0.1% Mannitol
Purity
Protein A or G purified

Alternate Names for Collagen V Antibody (NLI/64)

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol COL5A1
Entrez
Uniprot