Collagen V Antibody (MC5-HA)

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Product Details

Summary
Product Discontinued
View other related Collagen V Primary Antibodies

Order Details


    • Catalog Number
      NBP1-42216
    • Availability
      Product Discontinued

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Collagen V Antibody (MC5-HA) Summary

Immunogen
The antibody reacts with native human as wel as heat denatured human collagen type V in RIA, ELISA and immuno­blot assays.
Specificity
The antibody reacts with native human as wel as heat denatured human collagen type V in RIA, ELISA and immuno­blot assays. The antibody does not react with collagen types I, II, III and IV. Human plasma proteins do not interfere with binding to collagen.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
COL5A1
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The antibody is useful for staining of collagen V on cryostat sections of human tissue. The antibody can also be used for quantitative "sandwich" ELISA to determine concentration of collagen type V in human fluids.For staining of tissue sections it is advised to use acetone or ethanol fixed cryostat sections, results of staining paraffin embedded tissues, even after treatment with proteolytic enzymes or microwave, are less satisfactory. The antibody has to be dilut

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Ascites
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Collagen V Antibody (MC5-HA)

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol COL5A1