Collagen II Antibody

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Product Details

Summary
Product Discontinued
View other related Collagen II Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50135
    • Availability
      Product Discontinued

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Collagen II Antibody Summary

Immunogen
The antibody reacts with Human Collagen II.
Clonality
Polyclonal
Host
Goat
Gene
COL2A1
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Recommended for use in immunohistochemistry on frozen sections and for immunostaining of cultered human cells. Suitable for dot-blotting and ELISA on native human collagen type II. Use on paraffin sections is not tested.Antibodies can be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1:10.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1 ml IgG whole antisera (0.1 mg/ml)
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 1 ml sterilized water and with preservative if preferred.

Alternate Names for Collagen II Antibody

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

Antibodies to human collagen type II are raised in goats which are numerously immunized with extensively purified native collagen type II extracted from human cartilage into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, III, IV and V to remove cross-reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody is obtained by binding to immobilized native human collagen type II (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type II. Inhibition by other types of collagens is observed only at 20-50 times higher concentration. No inhibition is found with fibronectin, fribrinogen and laminin. Characteristic immunostaining pictures of frozen sections of human kidney, vertebrae, skin and ear are produced to certify batch quality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol COL2A1
Entrez