Collagen II Antibody



Product Details

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Collagen II Antibody Summary

The antibody reacts with Human Collagen II.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
1 ml IgG antiserum (0.1 mg/ml).
No Preservative
This product is unpurified. The exact concentration of antibody is not quantifiable.
Reconstitution Instructions
Reconstitute with 1 ml sterilized water and with preservative if preferred.


Application Notes
Recommended for use in immunohistochemistry on frozen sections and for immunostaining of cultered human cells. Suitable for dot-blotting and ELISA on native human collagen type II. Use on paraffin sections is not tested.Antibodies can be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1:10.

Reactivity Notes



Antibodies to human collagen type II are raised in goats which are numerously immunized with extensively purified native collagen type II extracted from human cartilage into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, III, IV and V to remove cross-reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody is obtained by binding to immobilized native human collagen type II (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type II. Inhibition by other types of collagens is observed only at 20-50 times higher concentration. No inhibition is found with fibronectin, fribrinogen and laminin. Characteristic immunostaining pictures of frozen sections of human kidney, vertebrae, skin and ear are produced to certify batch quality.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product PDFs


Gene Symbol COL2A1

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