Collagen I alpha 1 Native Protein

Images

 
Western Blot: Collagen I alpha 1 Native Protein [NBP1-97266] - Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Concentration
1.0 mg/ml

Order Details

Collagen I alpha 1 Native Protein Summary

Description
Native COL1A1 protein.

Source: Human Placenta/i>

(Accession #: P02452)

Preparation
Method
Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Human Collagen type I reacts with anti-Collagen Type I. Reaction of Human Collagen type I with Rockland's anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA)
Protein/Peptide Type
Native Protein
Gene
COL1A1
Purity
SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:10000
  • Immunohistochemistry 1:100 - 1:500
Application Notes
Collagen type I is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.5 M Sodium Acetate, pH 4.5
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
SDS-PAGE

Alternate Names for Collagen I alpha 1 Native Protein

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1

Background

Collagen type I is the fibrillar collagen found in most connective tissues, including cartilage. Collagen type I is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Collagen I alpha 1 Native Protein (NBP1-97266). (Showing 1 - 8 of 8 FAQ).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

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Bioinformatics

Gene Symbol COL1A1
Entrez