Collagen I alpha 1 Antibody (MC1-HA)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42217
    • Availability
      Product Discontinued

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Collagen I alpha 1 Antibody (MC1-HA) Summary

Immunogen
The antibody reacts with native as well as with heat denatured human collagen types I, II, III, in RIA, ELISA.
Specificity
The antibody reacts with native as well as with heat denatured human collagen types I, II, III, in RIA, ELISA. The antibody does not react with other collagen types (IV and V). Human plasma proteins do not interfere with binding to collagen.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
COL1A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The antibody is useful for staining of interstitial collagens (I, II, III) on cryostat sections of human sections. The antibody can also used for quantative "sandwich" ELISA to determine concentrations of interstitial collagens in human fluid (in combinatFor staining of tissue sections it is advised to use acetone or ethanol-fixed cryostat sections, results of staining of paraffin embedded tissues even after treatment with proteolytic enzymes or microwave are less satisfactory. For ELISA dilute 1:1000, fo

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Ascites
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Collagen I alpha 1 Antibody (MC1-HA)

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Collagen I alpha 1 Antibody (NBP1-42217). (Showing 1 - 8 of 8 FAQ).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL1A1
Entrez