Cofilin [p Ser3] Antibody

Images

 
Western Blot: Cofilin [p Ser3] Antibody [NBP1-51420] - Extracts from 3T3 paclitaxel cells.
Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP1-51420] - Paraffin-embedded human breast carcinoma tissue at 1:100.
Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP1-51420] - Human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related Cofilin Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51420
    • Availability
      Product Discontinued

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Cofilin [p Ser3] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.
Modification
p Ser3
Specificity
p-Cofilin (S3) pAb detects endogenous levels of Cofilin protein only when phosphorylated at S3.
Clonality
Polyclonal
Host
Rabbit
Gene
CFL1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-Cofilin (S3) pAb in extracts from 3T3 paclitaxel cells.
Immunohistochemistry (IHC) analyzes of p-Cofilin (S3) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cofilin [p Ser3] Antibody

  • 18 kDa phosphoprotein
  • CFL
  • cofilin 1 (non-muscle)
  • Cofilin, non-muscle isoform
  • cofilin-1
  • p18

Background

LIM-kinase 1 (LIMK-1) is a serine/threonine kinase containing LIM and PDZ domains. LIMK-1 phosphorylates Cofilin on Ser 3 both in vitro and in vivo. Cofilin is an Actin-depolymerizing factor and regulates Actin cytoskeletal reorganization. Phosphorylation of Cofilin on Ser 3 is known to block these activities. Phosphorylation of ADF/Cofilin proteins by LIMK-1 or other enzymes will tend to stabilize Actin filaments by inhibiting the ability of these proteins to sever and depolymerize older Actin filaments that have hydrolyzed their bound ATP and dissociated the phosphate. The rapid turnover of Actin filaments and the tertiary meshwork formation are regulated by a variety of Actin-binding proteins. Cofilin, therefore, is a terminal effector of signaling cascades that evokes Actin cytoskeletal rearrangement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Blogs on Cofilin.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

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Bioinformatics

Gene Symbol CFL1