COASY Overexpression Lysate (Native)


Western Blot: COASY Overexpression Lysate (Adult Normal) [NBL1-09341] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COASY.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

COASY Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215287 and based on accession number NM_001042531. The protein contains a C-terminal DDK tag.
Homo sapiens Coenzyme A synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 5, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for COASY

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for COASY Overexpression Lysate (Native)

  • bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
  • CoA synthaseUKR1
  • CoASY
  • Coenzyme A synthase
  • DPCK
  • FLJ35179
  • NBPbifunctional coenzyme A synthase
  • nucleotide binding protein
  • phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
  • pOV-2
  • PPAT


Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC and dephospho-CoA kinase (DPCK; EC, in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translation initiation codon, compared to variant 1. The resulting isoform (b, also known as CoASy gamma) is shorter at the N-terminus, compared to isoform a.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol COASY

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