COASY Antibody (3B3)

Images

 
Western Blot: COASY Antibody (3B3) [NBP2-01081] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: COASY Antibody (3B3) [NBP2-01081] Staining of COS7 cells transiently transfected by pCMV6-ENTRY COASY.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Human tonsil using anti-COASY mouse monoclonal antibody.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COASY mouse monoclonal antibody.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COASY mouse monoclonal antibody.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Human breast tissue using anti-COASY mouse monoclonal antibody.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Human Kidney tissue using anti-COASY mouse monoclonal antibody.
Immunohistochemistry-Paraffin: COASY Antibody (3B3) [NBP2-01081] - Staining of paraffin-embedded Human pancreas tissue using anti-COASY mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

COASY Antibody (3B3) Summary

Immunogen
Full length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
COASY
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for COASY Antibody (3B3)

  • bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
  • CoA synthaseUKR1
  • CoASY
  • Coenzyme A synthase
  • DPCK
  • FLJ35179
  • NBPbifunctional coenzyme A synthase
  • nucleotide binding protein
  • phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
  • pOV-2
  • PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COASY
Entrez