CNTF Antibody

Images

 
Western Blot: CNTF Antibody [NBP1-68875] - (0.5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

CNTF Antibody Summary

Immunogen
Peptide with sequence C-SIHDLRFISSHQ corresponding to internal region according to NP_000605.1.
Epitope
C-SIHDLRFISSHQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNTF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 17+28 kDa band bands observed in human, mouse and rat brain lysates (calculated MW of 22.9 kDa band according to human NP_000605.1 and 22.6 kDa band according to mouse NP_740756.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-68875.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CNTF Antibody

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, PEP-ELISA

Publications for CNTF Antibody (NBP1-68875)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CNTF Antibody (NBP1-68875) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CNTF
Entrez
Uniprot