CMT Antibody

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Western Blot: CMT Antibody [NBP1-69579] - This Anti-ICMT antibody was used in Western Blot of Fetal Heart tissue lysate at a concentration of 1ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CMT Antibody Summary

Immunogen
Synthetic peptides corresponding to ICMT(isoprenylcysteine carboxyl methyltransferase) The peptide sequence was selected from the middle region of ICMT. Peptide sequence GSNFNHVVQNEKSDTHTLVTSGVYAWFRHPSYVGWFYWSIGTQVMLCNPI.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ICMT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against ICMT and was validated on Western blot.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for CMT Antibody

  • EC 2.1.1.100
  • HSTE14
  • isoprenylcysteine carboxyl methyltransferase
  • Isoprenylcysteine carboxylmethyltransferase
  • MGC39955
  • MST098
  • pcCMT
  • PCCMTMSTP098
  • PPMTPCMT
  • Prenylated protein carboxyl methyltransferase
  • Prenylcysteine carboxyl methyltransferase
  • protein-S isoprenylcysteine O-methyltransferase
  • protein-S-isoprenylcysteine O-methyltransferase

Background

ICMT is the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ICMT
Uniprot