Recombinant Human CMBL Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human CMBL Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 245 of Human CMBL

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM
Protein/Peptide Type
Recombinant Protein
Gene
CMBL
Purity
>95% pure by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM EDTA
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
This product is useful for SDS-PAGE. The purity of this protein is > 95% by SDS - PAGE.
Theoretical MW
30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Alternate Names for Recombinant Human CMBL Protein

  • carboxymethylenebutenolidase homolog (Pseudomonas)
  • carboxymethylenebutenolidase homolog
  • carboxymethylenebutenolidase-like (Pseudomonas)
  • EC 3.1
  • FLJ23617

Background

Carboxymethylenebutenolidase homolog, also known as CMBL, is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15.2. Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CMBL

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