CLOCK Antibody

Product Discontinued

CLOCK Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human Clock.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLOCK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human,mouse,Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CLOCK Antibody

• BHLHE8
• circadian locomoter output cycles protein kaput
• Class E basic helix-loop-helix protein 8
• clock (mouse) homolog
• clock homolog (mouse)
• CLOCK
• EC 2.3.1.48
• hCLOCK
• KAT13D
• KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Background

Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. CLOCK, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the CLOCK gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. CLOCK contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that CLOCK may regulate circadian rhythmicity in combination with other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of CLOCK is seen in the hypothalamus and the eye.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLOCK
• Entrez:
  • 9575(Human)
  • 12753(Mouse)
  • 60447(Rat)
• Uniprot:
  • O15516()