cleaved ROCK1 Antibody (154C1465)


Western Blot: cleaved ROCK1 Antibody (154C1465) [NB100-56596] - Analysis of cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP more

Product Details

Reactivity Hu, Mu, Rt, Ca, Pm, RbSpecies Glossary
Applications WB, ICC/IF
0.5 mg/ml

cleaved ROCK1 Antibody (154C1465) Summary

This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21059388)
Read Publications using NB100-56596.

Reactivity Notes

Cross reacts with Chimpanzee.

Alternate Names for cleaved ROCK1 Antibody (154C1465)

  • EC 2.7.11
  • EC
  • MGC131603
  • MGC43611
  • p160 ROCK-1
  • p160ROCK
  • p160-ROCK
  • PRO0435
  • Renal carcinoma antigen NY-REN-35
  • Rho kinase
  • rho-associated protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil-containing protein kinase 1


The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cleaved ROCK1 Antibody (NB100-56596)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for cleaved ROCK1 Antibody (NB100-56596) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-56596AF405
Alexa Fluor (R) 488 Labeled NB100-56596AF488
Alexa Fluor (R) 647 Labeled NB100-56596AF647
Alexa Fluor (R) 700 Labeled NB100-56596AF700
Allophycocyanin Labeled NB100-56596APC
Biotin Labeled NB100-56596B
DyLight 350 Labeled NB100-56596UV
DyLight 405 Labeled NB100-56596V
DyLight 405LS Labeled NB100-56596V3
DyLight 488 Labeled NB100-56596G
DyLight 550 Labeled NB100-56596R
DyLight 650 Labeled NB100-56596C
DyLight 680 Labeled NB100-56596FR
DyLight 755 Labeled NB100-56596IR
HRP Labeled NB100-56596H
PE Labeled NB100-56596PE
PerCP Labeled NB100-56596PCP

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cleaved ROCK1 NB100-56596

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Contact Information

Product PDFs


Gene Symbol ROCK1

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