cleaved Retinoblastoma 1 Antibody (172C1094) [DyLight 680]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-Fr, IHC-P
Clone
172C1094
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

cleaved Retinoblastoma 1 Antibody (172C1094) [DyLight 680] Summary

Immunogen
A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.
Specificity
The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for cleaved Retinoblastoma 1 Antibody (172C1094) [DyLight 680]

  • OSRC
  • osteosarcoma
  • p105-Rb
  • pp110
  • pRb
  • RB
  • retinoblastoma 1
  • retinoblastoma suspectibility protein
  • retinoblastoma-associated protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-56598B
DyLight 350 Labeled NB100-56598UV
DyLight 405 Labeled NB100-56598V
DyLight 488 Labeled NB100-56598G
DyLight 550 Labeled NB100-56598R
DyLight 650 Labeled NB100-56598C
DyLight 755 Labeled NB100-56598IR
HRP Labeled NB100-56598H

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cleaved Retinoblastoma 1 NB100-56598FR

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Bioinformatics

Gene Symbol RB1