cleaved BAD Antibody

Images

 
Western Blot: cleaved BAD Antibody [NB100-56602] - Western blot analysis of Bad-cv using NB100-56602 at 1:500 against lysate of (A) untreated HeLa cells and (B) HeLa cells treated with TNFa for 2 hrs.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow-IC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
   

Biological Strategies

     

Order Details

cleaved BAD Antibody Summary

Immunogen
This antibody was developed by immunizing rabbits with a synthetic peptide containing Asp(cleavage site) of Bad.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250 - 1:500
  • Flow (Intracellular)
Application Notes
Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Positive Control
Bad Lysate (NBL1-07900)
Publications
Read Publication using
NB100-56602 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS with 0.1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for cleaved BAD Antibody

  • BBC2
  • BBC6
  • bcl2 antagonist of cell death
  • BCL2-antagonist of cell death protein
  • BCL2-associated agonist of cell death
  • Bcl-2-binding component 6
  • BCL2-binding component 6
  • BCL2-binding protein
  • Bcl2-L-8
  • BCL2L8bcl2-L-8
  • Bcl-2-like protein 8
  • BCL-X/BCL-2 binding protein
  • Bcl-XL/Bcl-2-associated death promoter

Background

The function of Bad, a proapoptotic member of the Bcl-2 family, is regulated primarily by rapid changes in phosphorylation that modulate its protein-protein interactions and subcellular localization. Human Bad, which is 43 amino acids shorter than its mouse counterpart, is cleaved by a caspase upon exposure of Jurkat T cells to anti-FAS antibody, tumor necrosis factor alpha (TNF-alpha), or TRAIL. Moreover, a truncated form of human Bad lacking the N-terminal 28 amino acids is more potent than wild-type Bad in inducing apoptosis. Bad plays a novel and important role in maintaining the apoptotic phenotype in response to various apoptosis inducers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cleaved BAD Antibody (NB100-56602)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow-IC.


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Flow-IC
(1)
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Positive Control Lysate(s)

Secondary Antibodies

 

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cleaved BAD NB100-56602

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Bioinformatics

Gene Symbol BAD
Entrez
Uniprot