Cleavage stimulation factor 2 Antibody

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Western Blot: Cleavage stimulation factor 2 Antibody [NB100-92330] - Western blot (WB) analysis of Cleavage stimulation factor 2(R46) antibody in extracts from 293 cells.

Order Details


    • Catalog Number
      NB100-92330
    • Availability
      Product Discontinued

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Cleavage stimulation factor 2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human CstF-64.
Specificity
Cleavage stimulation factor 2 (R46) detects endogenous levels of Cleavage stimulation factor 2 (R46) protein.
Clonality
Polyclonal
Host
Rabbit
Gene
CSTF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Cleavage stimulation factor 2 (R46) pAb in extracts from 293 cells.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse,.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cleavage stimulation factor 2 Antibody

  • betaCstF-64 variant 2
  • CF-1 64 kDa subunit
  • Cleavage stimulation factor 64 kDa subunit
  • cleavage stimulation factor subunit 2
  • cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD
  • cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
  • CSTF 64 kDa subunit
  • CstF-64

Background

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (also designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CSTF2
Entrez