Claudin-5 Antibody

Images

 
Immunohistochemistry-Paraffin: Claudin-5 Antibody [NB120-15106] - Formalin fixed paraffin embedded human colon with Claudin 5 antibody.
Immunohistochemistry: Claudin-5 Antibody [NB120-15106] - Man lung stained with Anti-Claudin 5 antibody, NB 120-15106

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Claudin-5 Antibody Summary

Immunogen
A synthetic peptide derived from the C-terminus of mouse Claudin-5.
Localization
Cell Membrane, cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLDN5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
For IHC: Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Positive Control
Claudin-5 Lysate (NBL1-09247)
Lung Lysate (NB820-59235)
Reviewed Applications
Read 2 Reviews rated 4
using
NB120-15106 in the following applications:

Publications
Read Publication using NB120-15106.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
IgG purified

Alternate Names for Claudin-5 Antibody

  • AWAL
  • AWALTransmembrane protein deleted in VCFS
  • BEC1
  • claudin 5
  • Claudin5
  • Claudin-5
  • CLDN5
  • CPETRL1
  • TMDVCF
  • TMVCF1
  • TMVCFTMDVCF
  • transmembrane protein deleted in velocardiofacial syndrome

Background

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Claudin-5 Antibody (NB120-15106)(1)

Reviews for Claudin-5 Antibody (NB120-15106) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 1 application: WB.

Reviews using NB120-15106:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Claudin-5 NB120-15106
Enlarge
reviewed by:
Jim Hsiao
WB Mouse 05/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary lung endothelial cells
SpeciesMouse
Lot012517

Comments

CommentsPrimary mouse lung endothelial cells were exposed to rabbit IgG (lane 2) or IL-1b (lane 3) for 24 hours. Lysates were analyzed by western blotting with an anti-mouse GAPDH antibody (1:2500) in 5% milk/TBST.
Western Blot Claudin-5 NB120-15106
Enlarge
reviewed by:
Anonymous
WB Human 06/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedHUVEC
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Claudin-5 Antibody (NB120-15106) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Claudin-5 Antibody (NB120-15106)

Discover related pathways, diseases and genes to Claudin-5 Antibody (NB120-15106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

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Research Areas for Claudin-5 Antibody (NB120-15106)

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Blogs on Claudin-5

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Contact Information

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Calculators

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Jim Hsiao
05/22/2017
Application: WB
Species: Mouse

Anonymous
06/09/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CLDN5
Entrez
Uniprot