Claudin-1 Antibody (2E2-H5)

Images

 
Western Blot: Claudin-1 Antibody (2E2-H5) [H00009076-M02] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M02), clone 2E2-H5.Lane 1: CLDN1 transfected lysate (Predicted MW: ...read more
Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody (2E2-H5) [H00009076-M02] - Huh7 cells were stained with CLDN1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody (2E2-H5) [H00009076-M02] - Analysis of monoclonal antibody to CLDN1 on Huh7 cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: Claudin-1 Antibody (2E2-H5) [H00009076-M02] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit ...read more
Sandwich ELISA: Claudin-1 Antibody (2E2-H5) [H00009076-M02] - Detection limit for recombinant GST tagged CLDN1 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00009076-M02
    • Availability
      Product Discontinued

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Claudin-1 Antibody (2E2-H5) Summary

Immunogen
CLDN1 (AAH12471, 1 a.a. ~ 212 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CLDN1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
This antibody is useful for ELISA, Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Claudin-1 Antibody (2E2-H5)

  • claudin 1
  • Claudin1
  • Claudin-1
  • CLD1
  • CLDN1
  • ILVASCSenescence-associated epithelial membrane protein
  • SEMP1
  • SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for Claudin-1 Antibody (H00009076-M02)

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Pathways for Claudin-1 Antibody (H00009076-M02)

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PTMs for Claudin-1 Antibody (H00009076-M02)

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Bioinformatics

Gene Symbol CLDN1
Entrez
Uniprot