Cited-2 Antibody (JA22)

Images

 
Immunocytochemistry/ Immunofluorescence: Cited-2 Antibody (JA22) [NB100-136] - The Cited-2 (JA22) antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Beta-tubulin and nuclei were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clone
JA22
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cited-2 Antibody (JA22) Summary

Immunogen
Human CITED 2 protein [UniProt# Q99967]
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CITED2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin
Application Notes
This CITED2 (JA22) antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at 29 kDa. Additional bands may be seen at 36 kDa representing p35srj, Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 19319572) the alternatively spliced version of CITED2, and at 70 kDa (unknown). Does not work well on mouse tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-136 in the following applications:

  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Cited-2 Antibody (JA22)

  • Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain2,cbp/p300-interacting transactivator 2
  • Cited2
  • Cited-2
  • MRG-1
  • MRG1melanocyte-specific gene 1-related gene 1
  • MSG1-related gene 1
  • MSG-related protein 1
  • P35srj

Background

CITED2 (Cbp/p300-interacting transactivator 2; also called MSG-related protein 1/ MRG1 or P35srj) is a CREBBP/EP300-interacting protein which is highly conserved in placental mammals with 95% identity shared between human and mouse protein. CITED2 is a transcriptional co-activator that stabilizes the interactions between DNA-binding proteins and the histone acetyltransferases CBP (CREB -binding protein) as well as p300. It is localized in the nucleus and can be induced upon hypoxic trigger wherein it functions as a negative regulator of HIF1 mediated signaling by competing with HIF1alpha for binding to CBP/p300. Additionally, CITED2 gets induced by several biological stimuli such as cytokines, serum and LPS in different cell type. CITED2 interacts with a diverse array of proteins including SMAD2, SMAD3, LHX1/2, ESR1/2, WT1, EP300, PPARA, TFAP2A, TFAP2B, TFAP2C, HNF4A etc and exerts versatile biological activities. CITED2 is a critical transcriptional cofactor involved in multiple organ development and mice lacking CITED2 die in utero with cardiac and aortic arch malformations, adrenal gland agenesis, small cranial and dorsal root ganglia, exencephaly, and neural crest and left-right patterning defects. Defects in CITED2 have been linked to ventricular septal defect type 2 (VSD2) and atrial septal defect type 8 (ASD8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-381
Species: Hu
Applications: WB, ChIP, IP
NBP1-76729
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ca, Ft, Mk, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
H00004435-M03
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC-P
NB100-77334
Species: Hu
Applications: WB, Flow, PEP-ELISA, KD
H00004212-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-52823
Species: Hu, Rt, Po, Bv, Ca, Eq, GP, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74359
Species: Hu, Mu, Ch
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22169
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-24597
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, IHC-P
NB100-1307
Species: Hu, Mu, Rt, Po
Applications: WB, IHC, IHC-P, PEP-ELISA
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP

Publications for Cited-2 Antibody (NB100-136)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-136 Applications Species
Lau WM, Doucet M, Huang D et al. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells. Biochem Biophys Res Commun 2013 Jun 27 [PMID: 23811274] (WB, Human) WB Human
Goncalves AM, de Sanctis D, McSweeney SM. Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans. J Biol Chem 2011 Sep 2 [PMID: 21733847]
Sengupta A, Molkentin JD, Paik JH. FoxO transcription factors promote cardiomyocyte survival upon induction of oxidative stress. J Biol Chem. 2010 Dec 15. [PMID: 21159781]
Yang Y, Dahly-Vernon AJ, Blomme EA et al. Liver transcriptomic changes associated with ritonavir-induced hyperlipidemia in sensitive and resistant strains of rats. Vet J 2010 Jul [PMID: 20547082]
Lau WM, Weber KL, Doucet M et al. Identification of prospective factors promoting osteotropism in breast cancer: a potential role for CITED2. Int J Cancer 2010 Feb 15 [PMID: 19642106] (WB, Mouse) WB Mouse
Haase M, Ansurudeen I, Schinner S. et al et al. Evidence for the involvement of endothelial cell products in adrenal CITED2 expression. Cell Tissue Res 2009 May [PMID: 19319572] (IHC-P, WB, Human) IHC-P, WB Human
Gonzalez YR, Zhang Y, Behzadpoor D, Cregan S, Bamforth S, Slack RS, Park DS. CITED2 signals through peroxisome proliferator-activated receptor-gamma to regulate death of cortical neurons after DNA damage. J Neurosci;28(21):5559-69. 2008 May 21. [PMID: 18495890]
Warnecke C, Weidemann A, Volke M, Schietke R, Wu X, Knaup KX, Hackenbeck T, Bernhardt W, Willam C, Eckardt KU, Wiesener MS. The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factors. Exp Cell Res;314(10):2016-27. 2008 Jun 10. [PMID: 18420194]
Haase, M et al. CITED2 is expressed in human adrenocortical cells and regulated by basic fibroblast growth factor. J Endocrinol;192(2):459-65. 2007 Feb. [PMID: 17283246]
Bamforth, S et al. Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway. Nat Genet;36(11):1189-96. 2004 Nov. [PMID: 15475956]
Show All 12 Publications.

Reviews for Cited-2 Antibody (NB100-136) (0)

There are no reviews for Cited-2 Antibody (NB100-136). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cited-2 Antibody (NB100-136) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Cited-2 Products

Bioinformatics Tool for Cited-2 Antibody (NB100-136)

Discover related pathways, diseases and genes to Cited-2 Antibody (NB100-136). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cited-2 Antibody (NB100-136)

Discover more about diseases related to Cited-2 Antibody (NB100-136).
 

Pathways for Cited-2 Antibody (NB100-136)

View related products by pathway.

PTMs for Cited-2 Antibody (NB100-136)

Learn more about PTMs related to Cited-2 Antibody (NB100-136).
 

Research Areas for Cited-2 Antibody (NB100-136)

Find related products by research area.

Blogs on Cited-2

There are no specific blogs for Cited-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cited-2 Antibody (JA22) and receive a gift card or discount.

Bioinformatics

Gene Symbol CITED2
Entrez
Uniprot