CIB1 Antibody (5A1F5E12)

Images

 
Western Blot: CIB1 Antibody (5A1H7E12) [NB110-89448] - recombinant protein(1) and A431 cell lysate (2).
Immunohistochemistry: CIB1 Antibody (5A1H7E12) [NB110-89448] - human thalamus(left) and glioma(right) tissue, showing membrane localization using anti-CIB1 antibody with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
5A1F5E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CIB1 Antibody (5A1F5E12) Summary

Immunogen
Ni-NTA purified truncated recombinant CIB1 expressed in E. Coli strain BL21 (DE3)
Specificity
Mouse Monoclonal to CIB1 (5A1H7E12)
Clonality
Monoclonal
Host
Mouse
Gene
CIB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
ELISA, Western Blot, Immunohistochemistry-Paraffin Although not tested this antibody may be useful in Frozen sections.
Positive Control
CIB1 Lysate (NBL1-09204)

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CIB1 Antibody (5A1F5E12)

  • calcium and integrin binding 1 (calmyrin)
  • Calcium- and integrin-binding protein
  • Calmyrin
  • CIB1
  • CIBcalcium and integrin binding protein
  • CIBP
  • DNA-dependent protein kinase interacting protein
  • DNA-PKcs-interacting protein
  • Kinase-interacting protein
  • KIP
  • KIP1
  • KIPcalcium and integrin binding, protein kinase interacting protein
  • PRKDCIP
  • SIP2-28
  • SIP2-28calcium and integrin-binding protein 1
  • Snk interacting protein 2-28
  • SNK-interacting protein 2-28

Background

CIB1(also designated calcium and integrin binding 1 or calmyrin),with 191-amino acid protein(about 21kDa), belongs to the calcium-binding protein family.CIB1 is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining.CIB1 is an EF-hand-containing protein that binds multiple effector proteins, including the platelet alpha(IIb)beta(3) integrin and several serine/threonine kinases and potentially modulates their function.CIB1 regulates platelet aggregation in hemostasis through a specific interaction with the alpha(IIb) cytoplasmic domain of platelet integrin alpha(IIb)beta(3). CIB1 is also ubiquitously expressed activating and inhibiting protein ligand of the InsP3R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CIB1 Antibody (NB110-89448)

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Blogs on CIB1.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

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Bioinformatics

Gene Symbol CIB1
Entrez