Chromata ChIP Kit

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Chromatin Immunoprecipitation: Chromata ChIP ChIP Kit [NBP1-71709] - ChIP analysis of HeLa Nuclear Extracts. Image provided via product review by verified customer.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - ChIP analysis of HEK293 and SH-SY5Y cells. Image courtesy of an anonymous customer review.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total ...read more
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - See notes for information on ChromataChIP Kit protocol information

Product Details

Summary
Applications ChIP

Order Details

Chromata ChIP Kit Summary

Description
This product contains all the necessary components to perform 25 Immunoprecipitation experiments including a positive control antibody (NB21-1023SS).
Specificity
This product contains all the necessary components to perform 25 Immunoprecipitation experiments including a positive control antibody (NB21-1023SS).
Kit Type
ChIP Kit

Applications/Dilutions

Application Notes
This ChromataChIP kit is a useful tool for Chromatin Immunoprecipitation.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-71709 in the following application:

Publications
Read Publications using
NBP1-71709 in the following applications:

  • 4 publications

Packaging, Storage & Formulations

Storage
Storage is content dependent.
Buffer
Dependent on kit component.

Kit Components

Components
  1. 10x Glycine
  2. IP Dilution Buffer
  3. H3 K4Me3 Positive Control Antibody
  4. Protein A/G Magnetic Beads
  5. IP Wash Buffer 1
  6. IP Wash Buffer 2
  7. IP Wash Buffer 3
  8. IP Wash Buffer 4
  9. IP Elution Buffer
  10. 5M NaCl
  11. Proteinase K
  12. Purification Columns
  13. Purification Column Tubes
  14. DNA Binding Buffer
  15. DNA Wash Buffer
  16. DNA Elution Buffer
  17. Human RPL30 Positive Control Primer Pair
  18. NBP1-71651: RPL30 primer

Notes

Click here for detailed information on the ChromataChIP kit View the complete ChromatChIP Kit components and protocol here.

Background

This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Chromata Kit (NBP1-71709)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


Filter By Application
ChIP
(4)
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Filter By Species
Human
(1)
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Showing Publications 1 - 5 of 5.
Publications using NBP1-71709 Applications Species
Yu HC, Hung MH, Chen YL et al. Erlotinib derivative inhibits hepatocellular carcinoma by targeting CIP2A to reactivate protein phosphatase 2A Cell Death Dis 2014 Aug 01 [PMID: 25077545] (ChIP) ChIP
Sheela Rani CS, Soto-Pina A, Iacovitti L, Strong R. Evolutionary conservation of an atypical glucocorticoid-responsive element in the Human tyrosine hydroxylase gene. J Neurochem 2013 May 7 [PMID: 23647419] (ChIP, Human) ChIP Human
Noto PB, Bukhtiyarov Y, Meng S et al. Regulation of Sphingomyelin Phosphodiesterase, Acid-like 3A Gene (SMPDL3A) by Liver X Receptors Mol Pharmacol 2012 Jul 24 [PMID: 22810003] (ChIP) ChIP
Ziyatdinova S, Viswanathan J, Hiltunen M, Tanila H. Reduction of Epileptiform Activity by Valproic Acid in a Mouse Model of Alzheimer's Disease is not Long-lasting after Treatment Discontinuation Epilepsy Research. 2015 Feb 13 (ChIP) ChIP
Fishman J, Homon A, Lamsa E et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB. 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120.

Reviews for Chromata Kit (NBP1-71709) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: ChIP.

Reviews using NBP1-71709:
Filter by Applications
ChIP
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation Chromata NBP1-71709
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reviewed by:
Corey.
ChIP Human 05/07/2013
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHeLa nuclear extract
SpeciesHuman
Lot031413
CommentsI generally had a good experience with the kit.

Primary Anitbody

Dilution Ratio5ug, overnight 4C

Details

Detection NotesqPCR
Fixation/Cross-Linkingsame as in the kit
DNS Sharing10 sec, X5 100-1000bp
Immunoprecipitation Detailsbeads included in the kit, 1h 4C
Crosslinking/Reversal Details1% formaldehyde, room temp 10min
DNA Prurification Detailskit columns

Comments

CommentsI generally had a good experience with the kit.
Chromatin Immunoprecipitation Chromata NBP1-71709
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reviewed by:
Jayashree Viswanathan
ChIP Human 10/19/2012
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHEK293 and SH-SY5Y cells
SpeciesHuman
CommentsThis kit is extremely easy to use and really WORKS! I have struggled with kits in the past but the beads in this kit are superior to some other vendors and the positive control antibody and primers are brilliant. Also I have tried various reverse-crosslinking parameters but incubation at 95C for 15 minutes as stated in the protocol works perfectly!

Primary Anitbody

Dilution RatioH3K4me3 antibody provided in the kit.

Details

Detection NotesRT-PCR using RPL30 positive control primers provided in the kit.
Fixation/Cross-LinkingAccording to protocol.
DNS SharingSonics Vibracell at 35% amplitude, 15sec ON 50sec OFF. Smear between 1000-100bp.
Immunoprecipitation DetailsBeads provided in the kit.
Crosslinking/Reversal DetailsParaformaldehyde to final concentration of 1% for 10 minutes at room temperature
DNA Prurification DetailsAccording to protocol.

Comments

CommentsThis kit is extremely easy to use and really WORKS! I have struggled with kits in the past but the beads in this kit are superior to some other vendors and the positive control antibody and primers are brilliant. Also I have tried various reverse-crosslinking parameters but incubation at 95C for 15 minutes as stated in the protocol works perfectly!

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar

FAQs for Chromata Kit (NBP1-71709). (Showing 1 - 6 of 6 FAQs).

  1. I received an inquiry about ChromataChIP Kit. Regarding phase 3(p.6), what size tube should be used? And what size tubes can be used for Magnetic tube separator rack (Cat# NBP1-72032)?
    • Generally this step is performed using 1.5mL tubes. The stand can hold eight 1.5-ml tubes or one 15-ml tube, and one 50-ml tube.
  2. I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?
    • As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.
  3. I am planning ChIP experiments using a rat cell line and also rat tissues. I am interested in your Chromata ChIP kit but I have some questions. The magnetic tube separator rack: is it possible to use a magnetic separator from Miltenyi Biotech (we already have one)? Is the positive control antibody (H3K4Me3) and primers (human RPL30) would work with rat samples? If no, could you provide a positive control working with the rat specie or give the primers sequences? Finally, what are the changes that have to be made to the protocol for ChIP assays starting from tissues? (Only an example using cells is given in the protocol)
    • 1. Yes, your magnetic rack will work just fine. 2. Unfortunately, our primers do not work with species other than human and we do not have any other primers available. You should be able to design your own to the same target in rat though and it should be just fine. 3. We are in the process of launching tissue specific protocols for use in ChIP. These are not available online yet but if you contact our Technical Team we can assist you with this novus@novusbio.com.
  4. Regarding the ChromataChIP Kit cat.#NBP1-71709: 1. What antibodies do you have? 2. Do you supply the qPCR primers for humans (positive and negative targets for each antibody)? 3. Do you supply the negative antibody? I like your guarantee, but hope never to hold you to it!
    • I am glad you are interested in our ChromataChIP Kits and would be happy to answer your questions. We have several ChIP grade epigenetics antibodies available and this link should take you to additional information about the kit itself and related products. I don't know if you saw this page first before inquiring further about the kit. Let me know if so and you still had additional questions and I would be happy to address those with the lab person who developed the kit. We are proud of our guarantee and of course prefer that no one would have to use it, but sometimes that is inevitable and as long as we can resolve any issues that arise are happy to do solid completely understand and know that is where your focus and attention will be. Let us know once you have more time on your hands if you are still interested in the lab visit and I can coordinate that with the lab.
  5. I wanted to know whether the H3K4me3 positive control antibody has cross reactivity with other species? I am using mouse tissue and would like to know whether this antibody will still work?
    • The positive control antibody in this kit is expected to cross-react with mouse, rat, c. elegans and most other mammals. It will work just fine for mouse.
  6. Do you know of anyone that has used this kit for plant tissues, preferably intact tissues? Also, do you have references where people have used your ChIP kit available?
    • We don't know of anyone using this kit on plants at this time. H3 is very well conserved, even in plants, as the immunogen to generate the antibody shares 100% similarity with tobacco, rice, maize and most other plants. This kit will almost certainly work with your plant. The main problem is that the primers almost certainly won't work on plants. Even one base pair difference can prevent the primers from working. Chances are there is some dissimilarity at the DNA level that doesn't effect the protein sequence because of the wobble rule. You can most likely use this kit on your plant, but you will need to design your own control primers.

Additional Chromata ChIP Kit Products

Chromata NBP1-71709

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Recent Reviews

4.5
5
1
4
1
3
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2
0
1
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Corey.
05/07/2013
Application: ChIP
Species: Human

Jayashree Viswanathan
10/19/2012
Application: ChIP
Species: Human