Chk1 Antibody

Images

 
Western Blot: Chk1 Antibody [NBP1-77753] - Detection of a bands corresponding to CHK1 in Saccharomyces cerevisiae lysates. Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 kDa. Lane 2 ...read more

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Chk1 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 312-327 of S. cerevisiae CHK1.
Specificity
This affinity purified antibody is directed against yeast CHK1 protein.Reactivity occurs against yeast CHK1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • ELISA 1:2000-1:10000
  • Immunoprecipitation 1:100
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a predominant band approximately 40-60 kDa in size corresponding - CHK1 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in yeast

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHEK1
Entrez