Chk1 Antibody


Western Blot: Chk1 Antibody [NB100-91696] - Extracts from MCF7 cells.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-91696] - Paraffin-embedded human breast carcinoma tissue.
Western Blot: Chk1 Antibody [NB100-91696] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat heart tissue lysate.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-91696] - Human breast carcinoma tissue.

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Chk1 Antibody Summary

Synthetic peptide, corresponding to amino acids 291-340 of Human Chk1.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Chk1 (N311) pAb in extracts from MCF7 cells.
Immunohistochemistry (IHC) analyzes of Chk1 (N311) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Chk1 Lysate (NBL1-09146)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC


Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 over expression, is due to phosphorylation by Chk1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol CHEK1