Chk1 Antibody

Images

 
Western Blot: Chk1 Antibody [NB100-91696] - Extracts from MCF7 cells.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-91696] - Paraffin-embedded human breast carcinoma tissue.
Western Blot: Chk1 Antibody [NB100-91696] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat heart tissue lysate.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-91696] - Human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related Chk1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91696
    • Availability
      Product Discontinued

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Chk1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 291-340 of Human Chk1.
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Chk1 (N311) pAb in extracts from MCF7 cells.
Immunohistochemistry (IHC) analyzes of Chk1 (N311) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Chk1 Lysate (NBL1-09146)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 over expression, is due to phosphorylation by Chk1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHEK1
Entrez
Uniprot