CHIP/STUB1 Antibody

Images

 
Western Blot: CHIP/STUB1 Antibody [NBP1-53139] - Lanes: 1 : 1ug insoluble STUB1 protein, 2: 1ug soluble STUB1 protein, 3: 1ug EPM2A protein, 4: 1ug insoluble PPP1R3C protein, 5: 1ug soluble PPP1R3C protein Primary ...read more
Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody [NBP1-53139] - Mouse Brain Slices, Red: Primary (1:400) Blue: DAPI Secondary: Anti-Rabbit IgG Alexa 594 (1:400)
Western Blot: CHIP/STUB1 Antibody [NBP1-53139] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-53139
    • Availability
      Product Discontinued

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CHIP/STUB1 Antibody Summary

Immunogen
Synthetic peptides corresponding to STUB1(STIP1 homology and U-box containing protein 1) The peptide sequence was selected from the N terminal of STUB1. Peptide sequence MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYG.
Clonality
Polyclonal
Host
Rabbit
Gene
STUB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against STUB1 and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for CHIP/STUB1 Antibody

  • Antigen NY-CO-7
  • Carboxy terminus of Hsp70-interacting protein
  • CHIP
  • CHIPSTIP1 homology and U box-containing protein 1
  • CLL-associated antigen KW-8
  • E3 ubiquitin-protein ligase CHIP
  • EC 6.3.2.-
  • heat shock protein A binding protein 2 (c-terminal)
  • HSPABP2
  • NY-CO-7
  • SDCCAG7
  • serologically defined colon cancer antigen 7
  • STIP1 homology and U-box containing protein 1
  • STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
  • STUB1
  • UBOX1

Background

STUB1 modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It has E3 ubiquitin-protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. STUB1 mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CHIP/STUB1 Products

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PTMs for CHIP/STUB1 Antibody (NBP1-53139)

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Bioinformatics

Gene Symbol STUB1
Entrez
Uniprot