| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC, IHC-P |
| Clone | 4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Janelia Fluor 549 |
| Immunogen | Synthetic human alpha-CGRP |
| Epitope | The epitope lies within residues 19-37 of human alpha-CGRP. |
| Specificity | This antibody binds human alpha-CGRP. Based on inhibition ELISA, this antibody cross-reacts up to 12% with human beta-CGRP and human amylin, while there is little (<4%) cross-reaction with rat alpha-CGRP. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CALCA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP3-00519AF350 | |
| Alexa Fluor 405 | NBP3-00519AF405 | |
| Alexa Fluor 488 | NBP3-00519AF488 | |
| Alexa Fluor 532 | NBP3-00519AF532 | |
| Alexa Fluor 594 | NBP3-00519AF594 | |
| Alexa Fluor 647 | NBP3-00519AF647 | |
| Alexa Fluor 700 | NBP3-00519AF700 | |
| Alexa Fluor 750 | NBP3-00519AF750 | |
| Biotin | NBP3-00519B | |
| DyLight 350 | NBP3-00519UV | |
| DyLight 405 | NBP3-00519V | |
| DyLight 488 | NBP3-00519G | |
| DyLight 550 | NBP3-00519R | |
| DyLight 594 | NBP3-00519DL594 | |
| DyLight 650 | NBP3-00519C | |
| DyLight 680 | NBP3-00519FR | |
| DyLight 755 | NBP3-00519IR | |
| FITC | NBP3-00519F | |
| HRP | NBP3-00519H | |
| Janelia Fluor 549 | NBP3-00519JF549 | |
| Janelia Fluor 646 | NBP3-00519JF646 |
Diseases for CGRP Antibody (NBP3-00519JF549)Discover more about diseases related to CGRP Antibody (NBP3-00519JF549).
| Pathways for CGRP Antibody (NBP3-00519JF549)View related products by pathway.
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PTMs for CGRP Antibody (NBP3-00519JF549)Learn more about PTMs related to CGRP Antibody (NBP3-00519JF549).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CALCA |
![ELISA: Human PTH ELISA Kit (Colorimetric) [NBP2-60629]](http://images.novusbio.com/images2/Human PTH ELISA Kit (Colorimetric)-ELISA-NBP2-60629-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32](http://images.novusbio.com/images2/Substance-P-Antibody-Immunocytochemistry-Immunofluorescence-NB300-187-img0005.jpg)
![Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).](http://images.novusbio.com/images2/Substance P Antibody-Immunohistochemistry-NB300-187-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.](http://images.novusbio.com/images2/Substance P Antibody-Immunocytochemistry-Immunofluorescence-NB300-187-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.](http://images.novusbio.com/images2/VIP-Antibody-(2)-Immunocytochemistry-Immunofluorescence-NBP1-05163-img0005.jpg)
![Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue. Dilution: 1:100](http://images.novusbio.com/images2/VIP-Antibody-(2)----BSA-Free-Immunohistochemistry-Paraffin-NBP1-05163-img0006.jpg)
![Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100](http://images.novusbio.com/images2/VIP-Antibody-(2)-Immunohistochemistry-Paraffin-NBP1-05163-img0002.jpg)
![Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Neuropeptide-Y-Antibody-Immunohistochemistry-NBP1-46535-img0001.jpg)
![Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)](http://images.novusbio.com/images2/Somatostatin-Antibody-(7G5)-Western-Blot-NBP2-37447-img0002.jpg)
![Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.](http://images.novusbio.com/images2/Somatostatin-Antibody-(7G5)-Immunohistochemistry-Paraffin-NBP2-37447-img0007.jpg)
![Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/Somatostatin-Antibody-(7G5)-Flow-Cytometry-NBP2-37447-img0003.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/images2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.](http://images.novusbio.com/images2/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Adrenomedullin/ADM [NBP2-78736] - Standard Reference Curve](http://images.novusbio.com/images2/Adrenomedullin-ADM-NBP2-78736-img0001.jpg)
![Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Vessel, endothelium](http://images.novusbio.com/images2/CRLR-Antibody-Immunohistochemistry-Paraffin-NLS6731-img0008.jpg)
![Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Analysis of anti-CALCRL / CGRP Receptor antibody with colon, submucosal plexus and submucosal vessels at 34 ug/ ml.](http://images.novusbio.com/images2/CALCRL-Antibody-Immunohistochemistry-Paraffin-NLS6731-img0003.jpg)
![Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Analysis of anti-CALCRL / CGRP Receptor antibody with human bone, osteosarcoma.](http://images.novusbio.com/images2/CALCRL-Antibody-Immunohistochemistry-Paraffin-NLS6731-img0004.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal to the top). E: Horizontal section (immunofluorescent preparation, posterior to the top). Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NB300-109-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NB300-109-img0007.jpg)
![Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/Tyrosine-Hydroxylase-Antibody-Simple-Western-NB300-109-img0008.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/images2/Thyroglobulin-Antibody-(2H11-+-6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0005.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).](http://images.novusbio.com/images2/Thyroglobulin-Antibody-(2H11-+-6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0003.jpg)
![Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.](http://images.novusbio.com/images2/Thyroglobulin-Antibody-(2H11-+-6E1)-Immunohistochemistry-NBP2-34294-img0004.jpg)