CES1 Antibody

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Western Blot: CES1 Antibody [NBP1-77764] - Analysis using the Biotin conjugate of NBP1-77764. Detection of 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. ...read more
Western Blot: CES1 Antibody [NBP1-77764] - Shows detection of hCE1 (arrowhead) transfected into U373 ug (glioblastoma) whole cell lysate (lane 2) as a 66 kDa band. Purified hCE1 (lane 4) is similarly detected. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

CES1 Antibody Summary

Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human CES1
Specificity
The antibody detects recombinant hCE1 in cell lysates and native CES1 present in human tissue or fluids in various assays.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CES1
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500 – 1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100

Reactivity Notes

Reactive in human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for CES1 Antibody

  • ACAT
  • acyl coenzyme A:cholesterol acyltransferase
  • Acyl-coenzyme A:cholesterol acyltransferase
  • Brain carboxylesterase hBr1
  • carboxylesterase 1 (monocyte/macrophage serine esterase 1)
  • carboxylesterase 1
  • carboxylesterase 2 (liver)
  • CEH
  • CES1A2
  • CES2EC 3.1.1.1
  • cholesteryl ester hydrolase
  • Cocaine carboxylesterase
  • EC 3.1.1
  • egasyn
  • HMSE1
  • HMSECES1A1
  • human monocyte/macrophage serine esterase 1
  • liver carboxylesterase 1
  • MGC117365
  • Monocyte/macrophage serine esterase
  • PCE-1
  • REH
  • Retinyl ester hydrolase
  • Serine esterase 1
  • SES1TGH
  • Triacylglycerol hydrolase

Background

CES1 is one of the enzymes responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. This enzyme is known to hydrolyze aromatic and aliphatic esters and is necessary for cellular cholesterol esterification. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia.Carboxylesterase 1 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. This enzyme is known to hydrolyze aromatic and aliphatic esters and is necessary for cellular cholesterol esterification. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. Three transcript variants encoding three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CES1