CENPH Antibody (2G4)

Images

 
Western Blot: CENPH Antibody (2G4) [NBP2-01449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: CENPH Antibody (2G4) [NBP2-01449] Staining of paraffin-embedded Human tonsil using anti-CENPH mouse monoclonal antibody.
Flow Cytometry: CENPH Antibody (2G4) [NBP2-01449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CENPH antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: CENPH Antibody (2G4) [NBP2-01449] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CENPH mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CENPH Antibody (2G4) [NBP2-01449] - Staining of paraffin-embedded Human lung tissue using anti-CENPH mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
2G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CENPH Antibody (2G4) Summary

Immunogen
Full length human recombinant protein of human CENPH(NP_075060) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CENPH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CENPH Antibody (2G4)

  • CENP-H
  • centromere protein H
  • ICEN35
  • Interphase centromere complex protein 35
  • kinetochore protein CENP-H
  • NNF1
  • PMF1

Background

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CENPH
Entrez