CEACAM8/CD66b Antibody (G10F5)

Images

 
Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells ...read more
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by ...read more
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was ...read more
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa ...read more
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Image from verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody ...read more
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
G10F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CEACAM8/CD66b Antibody (G10F5) Summary

Immunogen
Human peripheral blood cells.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready
Application Notes
It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.
Reviewed Applications
Read 1 Review rated 5
using
NB100-77808 in the following applications:

Publications
Read Publication using NB100-77808.

Reactivity Notes

Human. Predicited to cross react with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CEACAM8/CD66b Antibody (G10F5)

  • Carcinoembryonic antigen CGM6
  • carcinoembryonic antigen gene family member 6
  • carcinoembryonic antigen-related cell adhesion molecule 8
  • CD66b antigen
  • cd66b clone g10f5
  • cd66b G10F5
  • cd66b granulocyte marker
  • cd66b ihc
  • cd66b marker
  • cd66b neutrophil marker
  • cd66b neutrophils
  • CD66b
  • CD67 antigen
  • CD67
  • CEACAM8
  • CEACAM-8
  • CGM6
  • NCA-95
  • Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEACAM8/CD66b Antibody (NB100-77808)(1)

Review for CEACAM8/CD66b Antibody (NB100-77808) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-77808:
Filter by Applications
IHC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry CEACAM8/CD66b NB100-77808
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reviewed by:
Manuel Flores
IHC Human 06/09/2017
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Summary

ApplicationImmunohistochemistry
Sample TestedLiver cancer tissue
SpeciesHuman

Comments

CommentsIHC - Single staining of CEACAM-8/CD66b on FFPE section of human liver. Primary Ab is mouse IgM kappa monoclonal Ab from Novus Biologicals (NB100-77808). Secondary Ab is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. The cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal.

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FAQs for CEACAM8/CD66b Antibody (NB100-77808) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-77808AF405
Alexa Fluor 488 Labeled NB100-77808AF488
Alexa Fluor 647 Labeled NB100-77808AF647
Alexa Fluor 700 Labeled NB100-77808AF700
Allophycocyanin Labeled NB100-77808APC
DyLight 350 Labeled NB100-77808UV
DyLight 405 Labeled NB100-77808V
DyLight 488 Labeled NB100-77808G
DyLight 680 Labeled NB100-77808FR
DyLight 755 Labeled NB100-77808IR
PE Labeled NB100-77808PE
PerCP Labeled NB100-77808PCP

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Recent Reviews

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5
1
4
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1
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Manuel Flores
06/09/2017
Application: IHC
Species: Human

Bioinformatics

Gene Symbol CEACAM8
Entrez
Uniprot