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Mouse Cdk9 ELISA Kit (Colorimetric)

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Mouse Cdk9 ELISA Kit (Colorimetric) - Standard Curve Reference
Mouse Cdk9 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

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Mouse Cdk9 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse CDK9 in samples. No significant cross-reactivity or interference between Mouse CDK9 and analogues was observed.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5%
Intra-Assay
CV% < 5.47%
Spike Recovery
91-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CDK9

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Mouse Cdk9 ELISA Kit (Colorimetric)

  • C-2k
  • CDC2L4CTK1
  • CDC2-related kinase
  • Cell division cycle 2-like protein kinase 4
  • Cell division protein kinase 9
  • cyclin-dependent kinase 9 (CDC2-related kinase)
  • cyclin-dependent kinase 9
  • EC 2.7.11
  • EC 2.7.11.22
  • EC 2.7.11.23
  • PITALRE
  • serine/threonine protein kinase PITALRE
  • Serine/threonine-protein kinase PITALRE
  • TAK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Immunoprecipitation: Cdk7 Antibody [NB100-581] - Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 (lot NB100-581-2) used for IP at 6 ug per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody NB100-581-1 For blotting immunoprecipitated CDK7, NB100-581 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: Cdk7 Antibody [NB100-581] - Analysis using the Biotin conjugate of NB100-581. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.
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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
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Kit (Colorimetric)
NBP2-75181
Mouse Cdk9 ELISA Kit (Colorimetric)
Species: Mu
Applications: ELISA

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Bioinformatics

Gene Symbol CDK9