CDK2 Antibody

Product Discontinued

CDK2 Antibody Summary

• Immunogen: Human recombinant cdk2 protein.
• Specificity: Recognizes human Cdk2. Species cross-reactivity: mouse and rat.
• Isotype: IgG2a/IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 - 2 ug/ml

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4), 0.2% BSA and 40% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for CDK2 Antibody

• cdc2-related protein kinase
• CDK2
• cell devision kinase 2
• Cell division protein kinase 2
• cyclin-dependent kinase 2
• EC 2.7.11
• EC 2.7.11.22
• p33 protein kinase
• p33(CDK2)

Background

Cdk2 (Cyclin dependent kinase 2) belongs to the Ser/Thr protein kinase superfamily, CDC2/CDKX subfamily, and predicted molecular weight 34 kD. Cdk2 is most active during S phase and G2, and plays a critical role in cell division control in eukaryotes.Cdk2 has been shown to interacts with Cyclins A, B1, B3, D, or E. Cdk2 associates with Cyclin E to drive cells from G1 to S phase through phosphorylation of a limited number of targets including pRb. Cyclin A associates mainly with Cdk2 during S phase and G2, and has been implicated in the control of S phase. The Poly66332 antibody has been shown to be useful for western blotting, immunoprecipitation, and immunohistochemistry of human, mouse, and rat Cdk2 protein. Cyclins A, B1, B3, D, or E.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK2
• Entrez:
  • 1017(Human)
• Uniprot:
  • P24941()